The Rainbow Friends (Blue, Green, Orange, Purple, Yellow and Cyan) are the titular main antagonists of Rainbow Friends. The Rainbow Friends are very distinguishable creatures that vary in size, behavior and appearance. Five friends appear in Chapter 1, and six appear in Chapter 2.
Two unused friends, Pink and Senior Yellow, appear in Chapter 1's game files, whilst one appears in Chapter 2's game files, Red.
History
Creation
The Rainbow Friends were conceptualized as cartoon characters for a TV show called "Rainbow Funtime Friends", as confirmed by the Hemlock Wood's Museum.
In the same room, a rusted, cylindrical incubator[1] is captioned with the words "THIS IS WHERE THEY WERE ACTUALLY MADE", implying that the machine was used in the creation of the monsters.
1991
A photo titled Blue's First Friend is hung up inside of Hemlock Wood's Museum, which depicts Blue standing next to an unknown child. At this point, both of his eyes were intact. He also doesn't appear to be drooling.
1992
According to the merchandise, portraits titled "Rainbow Friends", "Blueโs Arm" and "Scientist" were created in 1992.
Unknown
Animatronic versions of Blue, Green, and Orange are seen demolished in the finale of Chapter 2. Their date of construction and possible usage is currently unknown. Sometime after 1991, Blue started drooling and lost his left eye, which was replaced with a button.
Present
The friends appear during the game as antagonists to the players. The Scientist acts the ringleader of the monsters, intentionally letting them attack and chase players while prompting them to collect items.
In the Chapters
Chapter 1

The bus after it crashed.
The Rainbow Friends make their debut in Chapter 1. After Scientist causes the bus to crash, he drags away the unconscious players into Hemlock Woods. Once they're awake, the Scientist tasks them to collect different items for him. Whilst doing so, the Rainbow Friends, Blue, Green, Orange, and Purple, roam the facillity with them, hunting them down and killing some of them.

Blue getting crushed by the metal door.
After five nights, the Scientist throws a goodbye party for the players. However, Blue is in the same room as them. He doesn't attack immediately though, and only reacts to popped balloons. After reaching the end of the room, the players get to eat the cake, which was promised by Scientist. Above the cake is a balloon, which falls down and pops, causing Blue to start chasing the players through the vents. Although close, the players manage to escape Blue and exit the facillity.
Chapter 2

After unsuccessfully stopping the players, the Rainbow Friends reappear in Chapter 2. While the players and the Technician are entering ODD World, Scientist releases Blue and Green from the garage. Purple also enters ODD World, this time staying in its lair to catch players. Afterwards the Scientist leaves to get the other Rainbow Friends, Yellow and Cyan. After 4 more hours, they manage to enter Orange's Cavern Coaster and board the carts.

Scientist, being mad at his failures.
Mid-Ride, Cyan ambushes the players and nearly kills them, only failing due to a drop. Throughout the ride, Orange, Blue, and Yellow attempt to kill the players during the ride, but they're are able to avoid all the Friends and fall down the garbage shaft. As they're about to leave the room, Cyan falls down the garbage shaft and starts chasing down the players. They are able to crouch under some metal bars and escape from her though, causing her to retreat back to Odd World.